FastQCFastQC Report
Mon 6 Jun 2016
ERR1391165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482903
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA25620.17276922361071492No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA18320.12354145888166658No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15320.10331087063685217Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT3550.040.56222515
GACGTCA3550.039.9284422
GATGACG3600.039.3738819
GCCGATG3650.038.83451516
TGACGTC3700.038.30972321
CGATGAC3700.038.30972318
ATGACGT3800.037.89365820
TAAGCCG3800.037.89365813
CGTCATA3800.037.89365824
CCGATGA3750.037.79892317
GCGGTCT2750.037.6365636
ACGTCAT3950.037.0242623
GGCGGTC2800.036.15968335
AGATCGG2250.034.99900425
CGGTATT3050.033.9334624
AAGCCGA4250.033.8813914
CGGTCTC3000.033.75017537
ATTCGGT3000.033.7490421
TCGGTAT3000.033.7490423
GATCGGA2350.033.50968626
Produced by FastQC (version 0.11.5)