FastQCFastQC Report
Mon 6 Jun 2016
ERR1391163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710464
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA30030.42268151517881275No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA20130.28333596072425904No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT17350.24420660300873792Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCCGTC14970.210707368705522Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCC8000.11260246824610395Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTC2950.044.236821
TAAGCCG2950.044.236813
CGTCATA2950.044.236824
AGCCGAT3050.042.78641515
GACGTCA3050.042.78641522
ATGACGT3050.042.78641520
CCGATGA3150.041.42811617
GCCGATG3150.041.42811616
TGCCGTC1700.041.0289645
ACGTCAT3200.040.78080423
GATCGGA2000.040.49955726
GATGACG3300.040.22683319
AAGCCGA3250.040.15340814
ATGCCGT1750.039.85670544
ATCGGAA2050.039.5117627
CGATGAC3350.038.95479618
TCGGAAG2200.037.84049628
AGATCGG2150.037.67400425
TTCGGTA2500.036.89959322
TCGGTAT2500.036.89959323
Produced by FastQC (version 0.11.5)