FastQCFastQC Report
Mon 6 Jun 2016
ERR1378584.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378584.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301361
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAAGATATCTCGTATGCC17450.5790397563055605Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1950.041.5384644
TATCTCG1950.041.5384639
ATCTCGT2000.040.50000440
CCGTAAG2000.040.50000431
TCGTATG2000.040.50000443
CGTAAGA2000.040.50000432
CTCGTAT2050.039.51219642
TCTCGTA2050.039.51219641
ACCGTAA2050.039.51219630
GACCGTA2050.038.41463529
GAGACCG2050.038.41463527
AGACCGT2050.038.41463528
GCGGTTC2150.037.674428
AGAGCGG2200.036.818185
CGGTTCA2250.036.0000049
GAGCGGT2250.036.0000046
CCGAGAC2200.035.79545625
AAGAGCG2300.035.217394
AGCGGTT2300.035.217397
CGGAAGA2200.033.751
Produced by FastQC (version 0.11.5)