FastQCFastQC Report
Mon 6 Jun 2016
ERR1378583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348251
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACAAATCTCGTATGCC19300.5541979778952537Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2300.042.0648141
ACCGATA2250.041.99959630
GACCGAT2200.041.93141629
GCGGTTC2400.041.2496078
CTCGTAT2350.041.1698242
GAGACCG2300.041.08656327
CGATACA2300.041.08656332
AGACCGA2250.040.99960328
CGTATGC2400.040.31211544
CGGTTCA2400.040.3121159
CGGAAGA2350.040.223931
CCGATAC2350.040.2123831
AGCGGTT2500.039.599627
GAGCGGT2500.039.599626
TCGTATG2450.039.4894243
AGAGCGG2550.038.823165
ATCTCGT2500.038.69962740
TGCCGAG2450.038.5710623
AAGAGCG2600.038.0765574
ATGCCGA2550.037.94081522
Produced by FastQC (version 0.11.5)