FastQCFastQC Report
Mon 6 Jun 2016
ERR1378582.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378582.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290234
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCC21920.7552526581999351Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2400.043.12499641
GACCGAT2350.043.08510629
ACCGATT2350.043.08510630
CTCGTAT2500.042.342
CCGATTA2400.042.18749631
AGACCGA2450.041.3265328
CGTATGC2550.040.58823444
TCGTATG2550.040.58823443
CGATTAA2500.040.49999632
ATCTCGT2550.039.70588340
GAGACCG2600.038.94230727
GTATGCC2800.036.96428745
GATCTCG2750.036.81818439
GCGGTTC2950.035.8474588
CGGAAGA2850.035.5263181
CGAGACC2900.034.91379526
AGAGCGG3050.034.672135
CCGAGAC3000.034.50000425
CGGTTCA3100.034.1129049
GAGCGGT3100.034.1129046
Produced by FastQC (version 0.11.5)