FastQCFastQC Report
Mon 6 Jun 2016
ERR1378578.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378578.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395634
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCC16840.42564592527436973Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1850.043.7831744
TCGTATG1850.043.7831743
GAGACCG1900.042.6309827
CTCGTAT1850.042.5669742
TCTCGTA1850.042.5669741
AGACCGA1950.041.5378828
CCGAGAG2000.040.4994331
GACCGAG2050.039.51164229
GAGCGGT2050.039.5116426
CGAGAGT2050.039.51164232
ATGCCGA2050.039.51164222
GCGGTTC2100.038.5708858
CCGAGAC2100.038.57088525
TGCCGAG2100.038.57088523
CGGTTCA2150.037.673899
ACCGAGA2150.037.6738930
ATCTCGT2100.037.49947440
AGCGGTT2200.036.8176657
AATGCCG2200.036.81766521
CGGAAGA2100.036.4326631
Produced by FastQC (version 0.11.5)