FastQCFastQC Report
Mon 6 Jun 2016
ERR1378577.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378577.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306240
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGTAATCTCGTATGCC17890.5841823406478579Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAGT2100.042.85527832
CCGGCAG2150.041.85864331
GACCGGC2150.041.85864329
ACCGGCA2200.040.9073130
CGTATGC2150.040.81217644
CTCGTAT2150.040.81217642
TCTCGTA2150.040.81217641
GAGACCG2300.040.1069527
AGACCGG2250.039.9982628
GTATGCC2200.039.8846345
GCGGTTC2450.039.4945268
CCGAGAC2250.038.99830225
CGGTTCA2500.038.698319
AAGAGCG2450.038.5823484
AATGCCG2350.038.29620421
ATGCCGA2350.038.29620422
TGCCGAG2350.038.29620423
ATCTCGT2300.038.15051340
GAGCGGT2550.037.945726
AGAGCGG2600.037.2220735
Produced by FastQC (version 0.11.5)