FastQCFastQC Report
Mon 6 Jun 2016
ERR1378575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300048
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGATGATCTCGTATGCC18630.6209006558950567Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1950.042.691243
CGTATGC2050.040.60870444
AGACCGG2050.040.60870428
ATCTCGT2100.039.64182740
ACCGGTG2100.039.64182730
CTCGTAT2100.039.64182742
GACCGGT2100.039.64182729
GAGACCG2100.039.64182727
TCTCGTA2100.039.64182741
CCGGTGA2100.039.64182731
GCGGTTC2150.038.7199258
GAGCGGT2150.038.7199256
CGGTGAT2150.038.71992532
AGCGGTT2200.037.8399287
CCGAGAC2250.036.99904325
AAGAGCG2300.036.200754
AGAGCGG2300.036.200755
CGGTTCA2300.036.1947149
TTCGTAG250.002104900335.9990714
TCGTAGA250.002104900335.9990715
Produced by FastQC (version 0.11.5)