FastQCFastQC Report
Mon 6 Jun 2016
ERR1378571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361382
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTACGAATCTCGTATGCC20570.5692037788268369Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT1800.044.9993129
CGGTACG1900.042.63092432
ATCTCGT1950.041.53782340
CGTATGC1950.041.53782344
TCTCGTA1950.041.53782341
AATCTCG1950.041.53782339
GTACGAA1950.041.53782334
ACCGGTA2000.040.4993830
AGACCGG2000.040.4993828
ACGAATC2000.040.4993836
CTCGTAT2050.039.5115942
GAGACCG2050.039.5115927
TACGAAT2050.039.5115935
CGAATCT2050.039.5115937
CCGGTAC2050.039.5115931
GGTACGA2250.037.99941633
GTATGCC2200.036.81761645
ATGCCGA2350.036.38242322
CCGAGAC2250.035.99944725
TCGTATG2250.035.99944743
Produced by FastQC (version 0.11.5)