FastQCFastQC Report
Mon 6 Jun 2016
ERR1378569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339768
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCC18250.5371312189494007Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1900.039.0783144
CTCGTAT1900.039.0783142
AGACCGA1900.039.0783128
TCGTATG1900.039.0783143
ATCTCGT2000.038.24937440
TCTCGTA2000.038.24937441
GACCGAG1950.038.07629829
ACCGAGT2000.037.12439330
GAGACCG2000.037.12439327
GTATGCC2000.037.12439345
CGAGTAG2050.036.2189232
CATCTCG2050.036.2189239
AAGAGCG2350.035.430174
CCGAGTA2100.035.35656431
AGAGCGG2300.035.2220045
CGGTTCA2200.034.7721569
CGAGACC2150.034.5343226
AGCGGTT2300.034.2385757
ATCGGAA401.5575197E-533.74944719
CGGAAGA2350.033.5150221
Produced by FastQC (version 0.11.5)