FastQCFastQC Report
Mon 6 Jun 2016
ERR1378565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305078
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCC19770.6480309953520084Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2050.042.80409641
CTCGTAT2100.041.7849542
TATCTCG2100.041.7849539
ATCTCGT2200.040.90834440
GACCGCA2250.039.9992729
ACCGCAG2300.039.12972630
CGCAGAG2300.039.12972632
CGTATGC2250.038.9992944
GAGACCG2350.038.29717327
AGACCGC2350.038.29717328
CCGCAGA2350.038.29717331
TCGTATG2300.038.1514843
CCGAGAC2450.036.73402825
GTATGCC2400.036.56183245
GCGGTTC2550.035.2934768
GAGTATC2500.035.0993636
AAGAGCG2650.034.8163954
AGAGCGG2600.034.6204265
CGGTTCA2600.034.6147549
AGCGGTT2600.034.6147547
Produced by FastQC (version 0.11.5)