FastQCFastQC Report
Mon 6 Jun 2016
ERR1378563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319998
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCAGTATCTCGTATGCC25570.7990674941718385Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2800.042.58854742
CGAGCAG2900.041.89582432
ATCTCGT2800.041.7849940
ACCGAGC2950.041.18572630
GACCGAG2900.041.11997629
TCGTATG2900.041.11997643
TCTCGTA3000.040.499341
CCGAGCA3000.040.499331
CGTATGC2950.040.42302744
AGACCGA3000.039.7493128
TATCTCG2950.039.66032839
GAGACCG3050.039.09768327
GTATGCC3100.038.46707545
CGAGACC3250.038.07626326
CAGTATC3300.035.4539336
CCGAGAC3450.035.21677825
GCGGTTC3650.035.1363758
AGCGGTT3650.035.1363757
CGGTTCA3650.034.5199479
AGAGCGG3800.033.754695
Produced by FastQC (version 0.11.5)