FastQCFastQC Report
Mon 6 Jun 2016
ERR1378561.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378561.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335557
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGCAATCTCGTATGCC20810.6201628933385386Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGC2650.044.99925232
CGTATGC2750.043.3629244
CTCGTAT2750.043.3629242
ATCTCGT2800.042.5885840
CCGAGTG2800.042.5885831
TCTCGTA2850.041.8414141
GAGACCG2950.041.1857627
GACCGAG3000.040.4993329
ACCGAGT2950.040.4230630
TCGTATG2950.040.4230643
CGAGACC3000.039.7493426
AATCTCG3050.039.0977139
CCGGGCA356.229404E-638.5765381
AGACCGA3150.038.5707928
CCGAGAC3100.038.46710625
GTATGCC3200.037.26500745
GCGGTTC3450.035.868978
AGAGCGG3500.035.3618285
CGGTTCA3500.035.3565569
AGCGGTT3500.035.3565567
Produced by FastQC (version 0.11.5)