FastQCFastQC Report
Mon 6 Jun 2016
ERR1378560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244953
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCC36721.499063085571518Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC2670.10900050213714467Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCT207.023351E-444.9987714
CTCGTAT3850.044.41437542
TCTCGTA3850.044.41437541
TCGTATG3850.044.41437543
ACCGGTA3950.043.2899630
CGTATGC3950.043.2899644
CCGGTAG3950.043.2899631
CGGTAGC3950.043.2899632
GACCGGT4000.042.74883729
AGACCGG4050.042.22107328
CGAGACC4000.042.1863526
GAGACCG4050.041.6655327
CCGAGAC4050.041.6655325
AATCTCG4050.041.6655339
GTATGCC4200.040.71317745
ATCTCGT4150.040.6615440
GGTAGCA4300.039.7663633
AGCGGTT4350.039.3092737
AATGCCG4350.039.30927321
GAGCGGT4400.038.8705146
Produced by FastQC (version 0.11.5)