FastQCFastQC Report
Mon 6 Jun 2016
ERR1378557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323461
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGACGATCTCGTATGCC15820.48908523747839766Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGA2050.040.6097631
CGTAGAC2050.040.6097632
GACGATC1950.040.38461736
GACCGTA2050.039.51219629
AGACGAT2000.039.37535
TAGACGA2100.038.57142634
GTAGACG2200.037.84090833
ACCGTAG2200.037.84090830
AGACCGT2150.037.6744228
CGTATGC2050.037.31707444
GAGACCG2200.036.8181827
ATCTCGT2100.036.4285740
TCGTATG2100.036.4285743
TAAGACC250.002104875436.012
GTATGCC2150.035.58139445
TCTCGTA2150.035.58139441
ACGATCT2300.035.21739237
CTCGTAT2200.034.77272842
GATCTCG2250.034.039
AAGAGCG2350.033.510644
Produced by FastQC (version 0.11.5)