FastQCFastQC Report
Mon 6 Jun 2016
ERR1378555.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378555.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353125
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTAGATCTCGTATGCC17470.49472566371681415Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA1900.041.4472432
AAGAGCG2050.040.6096274
GACCGGT1900.040.2630329
ATCTCGT2000.039.37487440
ACCGGTG2000.039.37487430
TCTCGTA2000.039.37487441
CGGAAGA2100.038.5767671
TCGTATG2050.038.41451343
GAGACCG2000.038.24987827
AGAGCGG2250.037.999885
CGGTTCA2200.037.840799
GGTGTAG2100.037.4998833
CCGGTGT2100.037.4998831
GCGGTTC2250.036.9998868
CGTATGC2150.036.62779244
CTCGTAT2150.036.62779242
AGACCGG2100.036.42845528
CGAGACC2100.036.42845526
GAGCGGT2350.036.3828626
AGCGGTT2300.036.195547
Produced by FastQC (version 0.11.5)