FastQCFastQC Report
Mon 6 Jun 2016
ERR1378554.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378554.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139427
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGTAGATCTCGTATGCC26401.893463963220897Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC2480.17787085715105397Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT2450.044.99605640
CGTATGC2450.044.99605644
GATCGGA302.1530523E-644.99605618
CGACGTA2400.044.99605632
CGTAGAT2450.044.99605635
TCTCGTA2450.044.99605641
GACCGAC2300.044.9960529
CTCGTAT2500.044.09613442
GACGTAG2450.044.07776633
ACCGACG2450.044.07776630
ACGTAGA2450.044.07776634
GAGACCG2400.044.05863627
GTATGCC2550.043.23150345
TCGTATG2550.043.23150343
AGACCGA2400.043.1212228
GATCTCG2650.042.4491139
GTAGATC2600.042.4001336
CCGACGT2550.042.34922831
AGATCTC2750.040.90550638
CGAGACC2650.039.90216426
Produced by FastQC (version 0.11.5)