FastQCFastQC Report
Mon 6 Jun 2016
ERR1378553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329291
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGTACATCTCGTATGCC22850.6939151085210346Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGTA2700.043.33333632
GACCGGA2650.043.30188829
TCTCGTA2750.042.54545641
TCGTATG2750.042.54545643
AGACCGG2700.042.50000428
CGTATGC2800.041.78571344
CCGGAGT2800.041.78571331
CATCTCG2800.041.78571339
CTCGTAT2850.041.0526342
GAGACCG2800.040.9821427
AGATCGG501.0768417E-940.517
GATCGGA501.0768417E-940.518
TCGGAAG501.0768417E-940.520
ATCTCGT2950.039.6610240
ACCGGAG2950.039.6610230
GGAGTAC3000.039.033
AGAGCGG3150.037.8571435
GTATGCC3100.037.74193645
CGGTTCA3200.037.2656259
CGAGACC3100.037.0161326
Produced by FastQC (version 0.11.5)