FastQCFastQC Report
Mon 6 Jun 2016
ERR1378550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305469
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCATAGATCTCGTATGCC15940.5218205448016002Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA2150.042.90588432
GAGACCG2150.041.85939827
AGACCGG2150.041.85939828
GAGCGGT2250.041.005676
CCGGCAT2250.040.99895531
GACCGGC2200.040.90804729
AGAGCGG2300.040.1142435
CTCGTAT2300.040.10767442
CGGTTCA2300.040.1076749
CCGAGAC2200.039.88534525
AGCGGTT2400.039.3804447
CGTATGC2350.039.2543244
TCTCGTA2350.039.2543241
TCGTATG2350.039.2543243
CGAGACC2250.038.99900426
GCGGTTC2400.038.4428148
ATCTCGT2400.038.43652340
ACCGGCA2400.038.43652330
GTATGCC2450.037.652145
ATGCCGA2550.037.0578822
Produced by FastQC (version 0.11.5)