FastQCFastQC Report
Mon 6 Jun 2016
ERR1378549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412189
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGAGATCTCGTATGCC21770.5281557732011286Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2450.042.244944
TCTCGTA2400.042.18749641
ATCTCGT2500.040.49999640
TCGTATG2600.039.80769343
CTCGTAT2550.039.70588342
AGACCGT2550.039.70588328
GTATGCC2700.039.16666445
GACCGTG2650.039.05660229
CCGTGTG2700.038.33333231
GAGCGGT2750.037.6363646
ACCGTGT2750.037.63636430
GCGGTTC2700.037.58
AGCGGTT2800.036.9642837
CGTGTGA2800.036.96428332
GATCTCG2800.036.96428339
CGGTTCA2750.036.8181849
GAGACCG2700.036.66666427
CGGAAGA2750.035.181821
AATGCCG2900.034.91379521
CCGAGAC2850.034.73684325
Produced by FastQC (version 0.11.5)