FastQCFastQC Report
Mon 6 Jun 2016
ERR1378547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396319
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGACATCTCGTATGCC27480.6933808371539089Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG3100.041.3754925
GACCGGA3150.040.713629
ATCTCGT3100.040.64447840
CGTATGC3100.040.64447844
CTCGTAT3100.040.64447842
AGACCGG3100.040.64447828
TCGTATG3100.040.64447843
GAGCGGT3200.040.077456
CCGAGAC3200.040.0774525
GCGGTTC3150.039.9993258
CGGAAGA3100.039.9287571
AAGAGCG3250.039.465854
CATCTCG3200.039.37433639
TCTCGTA3200.039.37433641
AGCGGTT3300.038.8629847
CGGTTCA3250.038.7685789
CCGGATG3250.038.76857831
GAGACCG3250.038.76857827
CGGATGA3250.038.76857832
ACCGGAT3300.038.1811830
Produced by FastQC (version 0.11.5)