FastQCFastQC Report
Mon 6 Jun 2016
ERR1378545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276946
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCGAGATCTCGTATGCC14140.5105688473565244Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG1100.042.954231
CGATCGA1100.042.954232
ATCTCGT1150.041.08662840
CTCGTAT1150.041.08662842
GACCGAT1150.041.08662829
TCTCGTA1150.041.08662841
CGTATGC1200.039.37468744
ACCGATC1200.039.37468730
TCGTATG1200.039.37468743
GAGACCG1250.037.79969827
AGACCGA1250.037.79969828
TCGAGAT1250.037.79969835
GCGGTTC1300.036.3458638
GTATGCC1300.036.34586345
GAGCGGT1300.036.3458636
TCACGAG250.002104436735.9997115
CGGAAGA1300.034.6213571
CCGAGAC1300.034.6151125
AGAGCGG1400.033.749735
ATGCCGA1350.033.3330722
Produced by FastQC (version 0.11.5)