FastQCFastQC Report
Mon 6 Jun 2016
ERR1378544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330014
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGAGACATCTCGTATGCC19120.5793693600877539Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1950.042.69230744
CTCGTAT1950.042.69230742
TCTCGTA1950.042.69230741
ATCTCGT2000.041.62500440
CCGTGAG1950.041.5384631
GAGACCG2050.040.60975627
TCGTATG2050.040.60975643
CGTGAGA2050.039.51219632
GACCGTG2050.039.51219629
AGACCGT2050.039.51219628
CGAGACC2200.038.86363626
GCGGTTC2150.038.720938
ACCGTGA2100.038.5714330
AGCGGTT2200.037.8409087
CATCTCG2250.037.039
GAGCGGT2250.037.06
CCGAGAC2250.037.025
AGAGCGG2250.037.05
AAGAGCG2350.035.4255334
CGGTTCA2350.035.4255339
Produced by FastQC (version 0.11.5)