FastQCFastQC Report
Mon 6 Jun 2016
ERR1378533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361312
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTAGTATCTCGTATGCC27950.7735696572491364Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG3850.045.039
CTCGTAT3900.044.42307742
CGTATGC3950.043.8607644
TCTCGTA3950.043.8607641
TCGTATG3950.043.8607643
ATCTCGT4000.043.312540
ACCGTCT4050.042.7777830
GACCGTC4000.042.7529
GAGACCG4050.042.22222527
CGTCTAG4150.041.7469932
CCGTCTA4150.041.7469931
AGACCGT4100.041.70731728
AGCGGTT4450.039.43827
GAGCGGT4400.039.3756
AGAGCGG4400.039.3755
GCGGTTC4500.039.08
GTATGCC4450.038.93258745
CCGAGAC4350.038.79310625
CGGTTCA4550.038.5714269
AAGAGCG4500.038.54
Produced by FastQC (version 0.11.5)