FastQCFastQC Report
Mon 6 Jun 2016
ERR1378531.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378531.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344065
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCC18900.5493148097016551Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG2550.044.11650539
TCGTATG2550.044.11650543
GACCGGT2600.043.26811229
CCGGTAT2550.043.23417731
CGGTATC2650.042.45173632
ATCTCGT2700.041.6655940
ACCGGTA2700.041.6655930
CGTATGC2700.041.6655944
TCTCGTA2700.041.6655941
GTATGCC2800.040.98108745
AGACCGG2750.040.90803528
GAGACCG2850.040.26211527
CTCGTAT2800.040.17753642
AAGAGCG3050.037.627454
CGAGACC3050.037.6219826
GCGGTTC3050.036.8896568
AGCGGTT3050.036.8896567
AGAGCGG3150.036.4329265
CGGTTCA3100.036.2893879
CCGAGAC3100.036.28938725
Produced by FastQC (version 0.11.5)