FastQCFastQC Report
Mon 6 Jun 2016
ERR1378528.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378528.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400254
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTACTATCTCGTATGCC14000.3497778910391901Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACT1800.043.74902730
GACCGAC1750.043.71331429
ATCTCGT1850.042.56662440
CCGACTA1850.042.56662431
AGACCGA1850.041.35043328
CGTATGC1950.040.3837244
CTCGTAT1950.040.3837242
GAGACCG1900.040.2622627
TATCTCG2000.039.37412639
TCTCGTA2000.039.37412641
CGACTAC2000.039.37412632
TCGTATG2050.038.4137843
CGAGACC2150.036.62709426
GACTACT2200.035.79466233
AGCGGTT2350.034.467327
GTATGCC2300.034.2383745
GCGGTTC2400.033.7492528
GAGCGGT2450.033.064626
CCGAGAC2350.032.55246725
AAGAGCG2550.031.7679674
Produced by FastQC (version 0.11.5)