FastQCFastQC Report
Mon 6 Jun 2016
ERR1378520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348349
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGTAATCTCGTATGCC19710.5658118725760659Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT2450.042.250026
AGAGCGG2500.041.4050185
CCGATAG2500.041.39907531
GACCGAT2500.041.39907529
CGATAGT2500.041.39907532
GCGGTTC2450.041.331548
AAGAGCG2600.040.678014
GCCGAGA2600.040.6721724
TGCCGAG2600.040.6721723
AGCGGTT2550.040.5931557
ACCGATA2550.040.58732630
TCTCGTA2550.040.58732641
AGACCGA2550.040.58732628
TCGTATG2550.040.58732643
GAGACCG2600.039.80680527
CCGAGAC2600.039.80680525
CGGTTCA2700.039.165799
CTCGTAT2650.039.0557342
AATCTCG2650.039.0557339
ATCTCGT2750.038.45368640
Produced by FastQC (version 0.11.5)