FastQCFastQC Report
Mon 6 Jun 2016
ERR1378516.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378516.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385411
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATAGAATCTCGTATGCC23280.6040305025025233Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3150.042.14006442
GACCGTA3050.042.05184629
ATCTCGT3300.041.5881540
TCTCGTA3250.041.53570641
AGCGGTT3200.041.4870077
GAGCGGT3200.041.4870076
AGAGCGG3200.041.4870075
CGTATGC3250.040.84874344
GCGGTTC3200.040.7838368
GAGACCG3250.040.1563927
AGACCGT3250.040.1563928
CGGAAGA2950.039.6635321
AAGAGCG3350.039.634524
TCGTATG3350.039.6293843
CGTATAG3300.039.54796632
CCGTATA3250.039.4640431
AATCTCG3450.039.1278439
GTATGCC3400.039.04659345
CGGTTCA3350.038.952649
ACCGTAT3350.038.2860130
Produced by FastQC (version 0.11.5)