FastQCFastQC Report
Mon 6 Jun 2016
ERR1378514.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378514.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107834
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAAGAATCTCGTATGCC38530.347795788899781Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAAGAATCTCGTATGCCGTC33440.3018502772075961Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15490.13982239216344686Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4700.037.8331943
ATGCCGT4950.035.92242444
TGCCGTC5150.034.52737845
CGGAAGA8650.024.7313251
TGAACCG751.6994072E-623.99699210
CGTTAAG9500.021.78772532
ACCGTTA9650.021.682230
CCGTTAA9550.021.67365531
GACCGTT9650.021.44905929
TCTCGTA9500.021.3150441
TCGTATG9600.021.10158243
AGACCGT10050.021.04213728
CTCGTAT9650.020.98656342
CGTATGC9700.020.88403944
AGATCGG3100.020.32003225
ATCTCGT10000.020.24746140
GAGACCG10450.020.23669827
GATCGGA3150.019.99749226
GTATGCC10250.019.76343245
ATCGGAA3200.019.68503227
Produced by FastQC (version 0.11.5)