FastQCFastQC Report
Mon 6 Jun 2016
ERR1378512.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378512.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782713
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAACAATCTCGTATGCC48290.6169566622759556Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATAACAATCTCGTATGCCGTC43780.5593365639768344Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT16770.2142547779326522Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5650.039.042444
TGCCGTC5950.037.0738845
TATGCCG5900.037.0065543
ATCGGAA3600.027.4971127
AGATCGG3600.026.87217725
CGGAAGA9450.025.9778671
GATCGGA3800.025.4578526
TCGGAAG4150.024.9371428
CCGAGAT4250.022.76231422
CCGATAA11100.021.6869131
ACCGATA11300.021.30307230
CGATAAC11300.021.30307232
CTCGTAT11100.021.28426742
GACCGAT11350.021.20922729
CGTATGC11150.021.19694744
TCTCGTA11050.021.17695241
GAAGAGC12100.020.8309143
GTATGCC11350.020.82343345
AGACCGA11650.020.66306728
ATCTCGT11150.020.58079740
Produced by FastQC (version 0.11.5)