FastQCFastQC Report
Mon 6 Jun 2016
ERR1378511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783498
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAATTAGATCTCGTATGCCGTC47860.6108503148699805Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATTAGATCTCGTATGCC45630.5823882128607859Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT20710.2643274137266464Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8740.11155101863693336Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6700.037.28304745
ATGCCGT6750.037.00687844
TATGCCG6750.036.6734843
CGAATTA10900.022.9097929
GATCGGA4300.022.49700226
ATCGGAA4300.022.49700227
CTCGTAT11000.022.49699839
GACCGAA11150.022.39611626
CGTATGC11100.022.3000241
CCGAATT11200.022.29613528
GTATGCC11150.022.20001642
TCGTATG11200.022.0952740
AGACCGA11250.021.99706625
AGATCGG4400.021.98570625
AATGCCG16250.021.87400618
ACCGAAT11450.021.80931927
TCTCGTA11250.021.79709438
ATCTCGT11350.021.6050537
TCGGAAG4600.021.5188728
ATGCCGA16450.021.47130219
Produced by FastQC (version 0.11.5)