FastQCFastQC Report
Mon 6 Jun 2016
ERR1378510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724489
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGTATATCTCGTATGCCGTC48610.6709556666836902Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGTATATCTCGTATGCC45620.6296851988090917Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18040.24900309045409935Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8670.1196705540042706Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6300.040.36469745
TATGCCG6350.040.04686743
ATGCCGT6450.039.42598344
GTATGCC10750.023.65069242
CCGAAGT11050.023.41420628
CTCGTAT10850.023.2237439
GACCGAA11050.023.21060426
CGAAGTA11050.023.21060429
TATCTCG10900.023.11880536
TCTCGTA10900.023.1172138
CGTATGC11000.023.11157841
ACCGAAG11100.023.10605227
GAGACCG11050.023.00541524
ATCTCGT11100.022.7022537
TCGTATG11200.022.69887440
AGACCGA11250.022.5964325
TCGAAAT500.002265886622.49644712
AATGCCG15600.022.06382218
TGCCGAG15750.021.8536920
ATGCCGA15900.021.64752219
Produced by FastQC (version 0.11.5)