FastQCFastQC Report
Mon 6 Jun 2016
ERR1378506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144194
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAAGATATCTCGTATGCCGTC40300.35221299884460155Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAAGATATCTCGTATGCC29300.2560754557356532Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18940.16553136968031645Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5450.036.75197243
TGCCGTC5500.036.0086745
ATGCCGT6000.033.0079544
GATCGGA3650.025.27232726
CCGTAAG8200.024.4202128
TCGGAAG3800.024.27579728
GACCGTA8250.024.27114926
CGTAAGA8350.024.2509829
ACCGTAA8300.024.12493727
AATGCCG12500.023.75845318
TCTCGTA8300.023.58280438
CTCGTAT8400.023.56989539
TATCTCG8350.023.4415936
AGACCGT8650.023.40888225
ATCGGAA3850.023.37510327
CGTATGC8550.023.15841341
TGCCGAG12850.022.9362520
ATGCCGA13100.022.84202619
TCGTATG8700.022.75813740
GAGACCG8950.022.12146624
Produced by FastQC (version 0.11.5)