FastQCFastQC Report
Mon 6 Jun 2016
ERR1378505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875242
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCCGTC26840.3066580442894651Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCC24400.27878004026315006Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13220.15104393984749362Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2950.035.8517843
TGCCGTC3250.034.61956445
ATGCCGT3100.034.1170244
GTCCGGT300.00514931229.99676320
GATCGGA2450.026.62977826
ATCGGAA2350.025.84827227
TGACCGT450.001227092324.99730326
AGATCGG2700.022.49757225
TCGGAAG2800.022.49757228
TCCGGTC550.003938259620.4523421
CCGATTA5200.020.33434528
CGTATGC5300.019.95067841
AATGCCG8400.019.8192918
CTCGTAT5500.019.63424539
TCTCGTA5450.019.40157538
CGGAAGA5350.019.3691581
AGCGGTT9000.019.2556254
CGATTAA5500.019.22629529
ATGCCGA8900.019.2114119
GACCGAT5400.019.16569329
Produced by FastQC (version 0.11.5)