FastQCFastQC Report
Mon 6 Jun 2016
ERR1378503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756995
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATAACATCTCGTATGCC42890.5665823420233951Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAATAACATCTCGTATGCCGTC38390.5071367710486859Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT16560.2187597011869299Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4700.038.78092244
TGCCGTC4750.038.37270445
TATGCCG4950.036.8222943
GATCGGA3650.024.655426
ATCGGAA3850.023.37459827
TCGGAAG4000.023.06050128
AGATCGG4000.022.4980525
CGGAAGA9100.022.2714161
CGAATAA9450.020.71249232
CCGAATA9550.020.49560731
GCGCCTT550.0039372920.45277432
GACCGAA9600.020.38885929
CGTATGC9500.020.37069344
TCGTATG9650.020.2872443
TCTCGTA9550.020.26136441
GAGACCG9750.020.07518427
AGACCGA9750.020.07518428
CTCGTAT9650.020.05272742
GTATGCC9700.019.95068245
ATCTCGT9750.019.84574540
Produced by FastQC (version 0.11.5)