FastQCFastQC Report
Mon 6 Jun 2016
ERR1378500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044226
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGTGATCTCGTATGCCGTC40500.38784707524999373Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGTGATCTCGTATGCC25800.24707295164073678Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18570.17783506635536753Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4800.038.44273444
TATGCCG4850.037.5824443
TGCCGTC5000.036.00490645
GAGACCG7200.024.9974224
ACCGGAT7350.024.79454427
GACCGGA7350.024.79335826
CGGATGT7450.024.46290629
CTCGTAT7450.024.46173339
AGACCGG7500.023.99752425
TCGTATG7550.023.83859840
CCGGATG7650.023.8233528
TCTCGTA7700.023.3753338
ATCTCGT7850.022.92866737
CGTATGC7900.022.78573241
GTATGCC8250.022.09180342
GCGGTTC12450.020.4264265
AAGAGCG12500.020.1753081
AATGCCG12500.020.15791918
AGCGGTT12550.020.0843414
GAGCGGT12550.020.0843413
Produced by FastQC (version 0.11.5)