FastQCFastQC Report
Mon 6 Jun 2016
ERR1378499.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378499.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041088
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGTAATCTCGTATGCCGTC51190.49169714759943445Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGTAATCTCGTATGCC47170.4530836970553882Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT21690.2083397368906375Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7250.040.35128843
TGCCGTC7400.039.8374645
ATGCCGT7550.038.74792544
TTCGGTA300.00514905429.99759936
GAGCGTA11750.024.89162330
CGTAATC11750.024.89162333
GCGTAAT11750.024.89162332
CGAGCGT11750.024.7001529
AGCGTAA11850.024.68156831
CTCGTAT12000.024.37304939
TCGTATG12100.024.35755540
CGTATGC12100.024.17510241
ACCGAGC12200.024.15790427
CCGAGCG12100.023.98568328
TCTCGTA12250.023.8756438
AGACCGA12250.023.8756425
GTATGCC12400.023.77168542
GACCGAG12350.023.68231426
GAGACCG12400.023.5868224
ATCTCGT12550.023.30490737
Produced by FastQC (version 0.11.5)