FastQCFastQC Report
Mon 6 Jun 2016
ERR1378498.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378498.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759195
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCCGTC38320.5047451576999321Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCC29830.39291618095482717Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15500.20416362067716462Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5150.038.45256843
ATGCCGT5200.038.08283244
TGCCGTC5400.036.6723645
TCGTTAT551.12791895E-732.723738
TCTCGTA8050.024.31413738
CTCGTAT8200.023.86936639
CGTATGC8400.023.57198141
ACCGAGA8250.023.4535527
GTATGCC8450.023.43249942
TCGTATG8500.023.29159440
ATCTCGT8450.023.1631737
AGACCGA8450.023.1631725
CGAGAGT8400.023.03473729
GACCGAG8450.022.89692726
CCGAGAG8750.022.37047828
CGGAAGA6600.022.1786021
GAGACCG8800.021.98625424
CATCTCG9050.021.87608336
ATGCCGA12050.021.65740419
AATGCCG12100.021.56791118
Produced by FastQC (version 0.11.5)