FastQCFastQC Report
Mon 6 Jun 2016
ERR1378497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191069
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACTGATCTCGTATGCCGTC35920.30157782630561286Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACTGATCTCGTATGCC25480.2139254736711307Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT16640.1397064317852282Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4350.037.24933643
TGCCGTC4850.033.409245
ATGCCGT4950.032.73426444
CTCGTAT6650.024.01962339
TCGGAAG3200.023.90533628
TCTCGTA6700.023.84037238
CGTATGC6800.023.49174941
AGACCGA6950.023.30650325
TCGTATG6950.022.98280140
ATCTCGT7100.022.4972537
GAGACCG7250.022.34209624
AATGCCG11000.022.0882118
GACCGAG7350.022.03812226
ATGCCGA10950.021.98361419
ACCGAGA7400.021.89105427
ATCGGAA3100.021.77336127
AAGAGCG11650.021.459471
AGAGCGG11550.021.2445492
GTATGCC7650.021.17566342
GAGCGGT11600.021.146763
Produced by FastQC (version 0.11.5)