FastQCFastQC Report
Mon 6 Jun 2016
ERR1378495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569929
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTGAATCTCGTATGCC25650.4500560596144432Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTGAATCTCGTATGCCGTC20750.3640804380896568Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10070.17668867525604065Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2900.039.5749143
ATGCCGT2950.038.9007344
TGCCGTC3050.038.3630545
CGGGCGG352.8190235E-432.14204422
GATCGGA2050.026.34079626
ATCGGAA2150.025.1156427
TCGGAAG2400.022.4994328
AGATCGG2400.021.56195425
CGGAAGA4950.021.3781011
AATGCCG7800.021.34373918
CGTATGC5400.021.25132841
CTCGTAT5450.021.05451239
TCTCGTA5450.021.05451238
CGGTTCA8250.021.0031536
ATGCCGA8000.020.81014619
TGCCGAG8000.020.81014620
CCTTCGT654.2153147E-420.7832931
CCGAGAT2500.020.69947422
GCGGTTC8300.020.6036975
TCGTATG5600.020.49055340
Produced by FastQC (version 0.11.5)