FastQCFastQC Report
Mon 6 Jun 2016
ERR1378494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617748
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCTGAATCTCGTATGCC26150.42331177114292556Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCTGAATCTCGTATGCCGTC23630.3825184379390949Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11660.18875010522089913Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3400.039.71724343
ATGCCGT3800.035.5364844
TGCCGTC3900.034.6252945
CGGAAAC300.00514842429.99643914
TAACGGG300.00514842429.99643918
GATCGGA2500.026.99679626
AGATCGG2800.024.1042825
ATCGGAA2800.024.1042827
CGGAAGA5850.023.0947481
TCGGAAG2950.022.8786428
AATGCCG9350.021.4145718
CCGAGAT3200.021.09124622
TCGTATG6550.020.9517140
CGTATGC6500.020.77517144
CTCGTAT6500.020.76844642
TGCCGAG9700.020.64187820
TCTCGTA6600.020.45377241
ATGCCGA9800.020.4312519
GACCGAG6700.020.14686226
AATCTCG6700.020.14686236
Produced by FastQC (version 0.11.5)