FastQCFastQC Report
Mon 6 Jun 2016
ERR1378493.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378493.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786024
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCATGAATCTCGTATGCCGTC25350.32250923635919515Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCATGAATCTCGTATGCC24470.31131364945599627Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15150.19274220634484443Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3400.037.7239543
ATGCCGT3850.033.89912444
TGCCGTC3850.033.31465545
GATCGGA2800.026.51684626
ATCGGAA2950.025.1701327
AGATCGG2950.025.1669325
TCTCGTA5550.023.10869838
CGTATGC5600.022.9067441
TCGTATG5600.022.90236940
TCGGAAG3350.022.83640128
CTCGTAT5550.022.70328139
AATGCCG9150.022.6192118
ATGCCGA9100.022.49627919
TGCCGAG9150.022.37334820
ACCGGCA6050.022.3146227
AGACCGG5900.022.11639225
GTATGCC5850.021.92363542
GAGACCG6100.021.39126424
GCGGTTC9600.021.3340975
ATCTCGT6150.020.85418937
Produced by FastQC (version 0.11.5)