FastQCFastQC Report
Mon 6 Jun 2016
ERR1378492.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378492.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553021
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCCGTC36250.6554904786617507Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCC32650.5903934931946526Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18180.3287397766088449Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7790.14086264355241482Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4550.039.5704644
TGCCGTC4700.038.3075745
TATGCCG4850.037.12280343
GACGTCC300.00514770629.99674623
CTCGTGG406.1659777E-428.1219489
AGATCGG3450.022.8236125
ATCGGAA3600.021.87262527
GGATCGA8350.021.8239830
GTATGCC8250.021.82370842
AGACCGG8250.021.81581325
CGAATCT8250.021.81581334
ACCGGAT8300.021.68439527
TCGAATC8300.021.68439533
CGGATCG8450.021.56570629
GACCGGA8350.021.55454826
CGTATGC8400.021.43012241
CTCGTAT8400.021.42624939
GAGACCG8400.021.42624924
GATCGGA3700.021.28147326
GATCGAA8600.021.1895631
Produced by FastQC (version 0.11.5)