FastQCFastQC Report
Mon 6 Jun 2016
ERR1378490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035835
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACGTATCTCGTATGCCGTC59770.5770224022165692Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACGTATCTCGTATGCC52750.5092509907465957Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT26480.2556391703311821Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC12400.1197101855025173Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7300.038.53128443
TGCCGTC7350.037.96301345
ATGCCGT7300.037.91478344
TGCGTTA406.169056E-428.12240612
AGACGTA12650.022.5879431
GAGACGT12550.022.40937430
GACGTAT12750.022.23431632
ACGTATC12850.021.88619633
CTCGTAT12750.021.8813939
TCGTATG12750.021.8813940
CGTATCT12900.021.80136734
TATCTCG12800.021.79591836
CGTATGC12950.021.7192941
CGAGACG13050.021.55077729
AGACCGA12850.021.53497725
TCTCGTA13150.021.21579638
GAGACCG13100.021.12400424
GACCGAG13250.021.0546626
ACCGAGA13300.020.9755127
ATCTCGT13450.020.74258237
Produced by FastQC (version 0.11.5)