FastQCFastQC Report
Mon 6 Jun 2016
ERR1378489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806754
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCCGTC32460.4023531336689995Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCC22570.27976310002801347Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT16820.20848982465534724Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3850.039.74436643
ATGCCGT4050.037.7816844
TGCCGTC4550.033.6298545
CCAAACG507.1628456E-526.99609620
ACCGAGT6050.024.91684227
CGTATGC6150.024.87753541
TCTCGTA6150.024.87599438
TCGTATG6150.024.87599440
AGACCGA6150.024.8744525
CTCGTAT6200.024.6753839
GAGACCG6400.024.25430524
GACCGAG6400.023.90427626
ATCGGAA2850.023.6837227
TCGGAAG2900.023.27538128
GATCGGA3000.023.2480826
ATCTCGT6500.023.1903937
AATGCCG9850.022.83933418
CCGAGTA6700.022.49953728
CGAGTAG6750.022.33287229
CATCTCG6750.022.33148836
Produced by FastQC (version 0.11.5)