FastQCFastQC Report
Mon 6 Jun 2016
ERR1378488.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378488.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531290
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAGAATCTCGTATGCCGTC33760.6354345084605395Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAGAATCTCGTATGCC24090.45342468331796193Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18680.35159705622164916Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7440.14003651489770183Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4150.041.21042343
ATGCCGT4600.037.17896744
TGCCGTC4700.036.38792445
ATTGCCG300.00514611129.99843227
CGGCTAG6800.025.47900629
CTCGTAT6750.025.33200839
TCTCGTA6750.025.33200838
CGTATGC6850.024.96219641
GTATGCC6900.024.78364642
TCGTATG6900.024.78131340
ACCGGCT7050.024.57318327
CCGGCTA7200.024.3760228
AGACCGG7150.024.2272225
ATCTCGT7100.024.08324637
AATCTCG7200.023.74875836
GAGACCG7350.023.56797824
GCTAGAA7600.023.09307131
GGCTAGA7700.022.79316330
AATGCCG11950.022.02606418
GACCGGC7900.021.9271726
Produced by FastQC (version 0.11.5)