FastQCFastQC Report
Mon 6 Jun 2016
ERR1378487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891327
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAGAATCTCGTATGCCGTC41450.46503696174355763Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAGAATCTCGTATGCC29570.3317525442402171Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT16030.17984420981300914Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4900.039.03503843
ATGCCGT5050.037.87558444
TGCCGTC5500.035.1858145
ATCGGAA3500.025.07005527
GATCGGA3600.024.37229526
ACCGTCG8150.024.01708827
CGTATGC8000.023.90627941
AGACCGT8000.023.90359725
CCGTCGA8250.023.72597128
TCGGAAG3700.023.71491428
AGATCGG3800.023.68158325
CTCGTAT8150.023.46365439
GACCGTC8150.023.46365426
TCTCGTA8150.023.46365438
CGTCGAG8350.023.44182829
AATGCCG12200.023.23512618
TCGTATG8400.022.76660740
ATCTCGT8400.022.7653337
CGAGAAT8500.022.76345632
AATCTCG8500.022.49750336
Produced by FastQC (version 0.11.5)