FastQCFastQC Report
Mon 6 Jun 2016
ERR1378486.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378486.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893229
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGAGTATCTCGTATGCC33670.376947009109646Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACGAGTATCTCGTATGCCGTC29050.32522455047921645Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12530.14027757719464998Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3450.039.15001743
ATGCCGT3850.035.08051744
TGCCGTC4150.033.08698745
AGATCGG2800.025.71131125
CGGAAGA7950.025.49731
GATCGGA2850.025.26023726
ATCGGAA2850.025.26023727
CGAGTAT8250.024.816735
ACGAGTA8400.024.37217934
CTCGTAT8250.024.27807442
GACGAGT8450.024.22796633
TCGTATG8300.024.13857843
ACCGACG8500.024.08544730
TATCTCG8350.023.98060439
CGACGAG8550.023.944632
ATCTCGT8400.023.83786240
CGTATGC8500.023.83411644
TCTCGTA8450.023.70344541
GACCGAC8650.023.66778229
GTATGCC8800.023.5331845
Produced by FastQC (version 0.11.5)