FastQCFastQC Report
Mon 6 Jun 2016
ERR1378485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642681
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCC39830.6197475886170588Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCCGTC35410.5509731888759742Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT17430.27120764422785176Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7850.12214457872568195Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4000.038.25893444
TATGCCG4100.037.32578743
TGCCGTC4300.034.5429545
CGGAAGA8000.022.2429831
GTATGCC8250.021.00490445
AGCTCCG550.003936452320.45295335
AGATCGG3300.020.45136325
TCTCGTA8400.020.35872841
TCGTATG8450.020.24141143
CTCGTAT8450.020.2382642
AGACCGT8400.020.08772328
CGTATGC8650.020.03357944
CCGTGCA8500.019.85139731
CGTGCAG8450.019.70260832
GACCGTG8700.019.65364329
AATCTCG8750.019.54133839
ATCTCGT8800.019.43030540
ACCGTGC8850.019.32053230
ATCGGAA3500.019.28421427
GAGACCG8950.019.10465827
Produced by FastQC (version 0.11.5)