FastQCFastQC Report
Mon 6 Jun 2016
ERR1378484.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378484.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860695
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCAGTATCTCGTATGCC32730.3802740808300269Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCAGTATCTCGTATGCCGTC28460.330663010706464Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15120.17567198601130482Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4600.037.1861144
TATGCCG4850.035.269343
TGCCGTC4850.035.269345
CCTTCGT1052.3392204E-923.5928751
GATCGGA2750.022.08788526
CGTATGC8000.021.6583241
CGGAAGA6250.021.6196521
GTATGCC8050.021.52629742
CTCGTAT8000.021.37207439
TCTCGTA8050.021.23932838
AGACCGA8300.021.14168525
TATCTCG8200.021.12515836
TCGTATG8250.020.99712840
ATCTCGT8250.020.72443837
ATCGGAA2950.020.59040327
AATGCCG11300.020.50604418
CGCCCGG1009.0613867E-720.24722913
ATGCCGA11850.020.12382919
GACCGAG8800.019.94045326
ACCGAGC8800.019.94045327
Produced by FastQC (version 0.11.5)